| Congresso Brasileiro de Microbiologia 2023 | Resumo: 1180-2 | ||||
Resumo:Antimicrobial resistance is a top priority problem for global public health, with a complex dispersion pattern among humans, animals, and the environment. For this reason, the WHO has proposed the study of E. coli BLEE as an indicator microorganism of antimicrobial resistance in the three main axes (human, animal, and environment) following schemes such as the "Tricycle" protocol, which focuses on the study of poultry. In this context, this protocol was implemented for the first time in Paraguay, determining the prevalence of E. coli BLEE in 237 samples of chicken feces from intensive (16%) and family (84%) farming systems, from 5 departments and 18 cities in the eastern region of Paraguay. Bacterial colonies resistant to cefotaxime were isolated and the species and production of BLEE were confirmed by conventional microbiological and molecular methods. Genotypic detection was carried out by conventional PCR and the BLEE genes in question were: blaCTX-M together with its variants, blaTEM, blaSHV, and blaPER-2. The carriage of beta-lactam-resistant bacteria in the chickens studied was 37% (87/ 237), of which E. coli BLEE accounted for 26% (61/237). All of the intensively reared chickens showed positive resistance to E. coli BLEE. The most frequent bla genotypes were blaCTX-M and blaTEM with 93% and 82% carriage. The most frequently detected blaCTX-M variants were; blaCTX-M-1 with 82%, followed by blaCTX-M-2 with 35% and blaCTX-M-8/25 and blaCTX-M-9 with 26% and 21%, respectively. The distribution of some blaCTX-M variants had significant differences according to breeding type. The SHV gene was present in only 4% of BLEE isolates and did not detect PER-2 gene carriage in any of the samples. These results are the first of their kind to confirm the active circulation of BLEE genes in chickens in Paraguay, obtaining valuable epidemiological data, comparable with other countries that have also implemented the tricycle protocol. Among the future perspectives, we hope to perform a deeper epidemiological analysis by sequencing the complete genome of these isolates. Palavras-chave: chicken feces, E. coli BLEE, Tricycle protocol Agência de fomento:Instituto de Investigaciones en Ciencias de la Salud, Universidad Nacional de Asunción, San Lorenzo, Paraguay | |||||